human t lymphoblast jurkat cells  (ATCC)

Journal: ACS Biomaterials Science & Engineering

Article Title: Delivery of Circular RNAs into Splenic Immune Cells via Intravenous Administration of Polyaspartamide Derivative Polyplexes

doi: 10.1021/acsbiomaterials.5c02147

Figure Lengend Snippet: Luminescence intensities of (A) Jurkat, (B) NK-92 mi, and (C) RAW 264.7 cells transfected with FLuc linear or circular RNA-loaded PAsp­(DET/CHE) polyplexes ( N / P = 2.8, 100 ng RNA/well) for 24 h. Results are expressed as mean ± SD ( n = 4). (D) Cellular uptake of naked Cy5-RNA and Cy5-RNA-loaded PAsp­(DET/CHE) polyplexes ( N / P = 2.8, 500 ng Cy5-RNA/well) into Jurkat, NK-92 mi, and RAW 264.7 cells after incubation for 4 h, measured using flow cytometry.

Article Snippet: Human natural killer NK-92 mi and human T lymphoblast Jurkat cells were obtained from the American Type Culture Collection (Manassas, VA, USA).

Techniques: Transfection, Incubation, Flow Cytometry

Journal: bioRxiv

Article Title: Human cell F-actin density differentially influences trogocytosis and phagocytosis by Entamoeba histolytica

doi: 10.64898/2026.03.17.712427

Figure Lengend Snippet: A, Western blotting analysis of dCas9 expression among Jurkat T cell clonal lines that were stably transduced with a construct for expression of HA-tagged dCas9. Wild-type Jurkat T cells were used as a control. Western blots were probed with HA antibody (top) or alpha-tubulin (α-tubulin) antibody as a loading control (bottom). The clonal line “2D10” was used for subsequent experiments in which constructs for sgRNA expression were stably transduced. B, RT-qPCR analysis of CRISPRi knockdown mutants. Jurkat 2D10 cells stably transduced with a construct for expression of sgRNA corresponding to the ANPEP gene, which is not expressed in Jurkat cells, were used as a control. Expression of individual target genes in knockdown mutants was compared to the expression of the same gene in ANPEP control cells using unpaired, two-tailed t-tests with Welch’s correction, p<0.0001 (****). C, Western blotting analysis of ANPEP control, ROCK1 and MYPT1 knockdown mutants. Blots were probed with ROCK1 antibody (top) or α-tubulin antibody as a loading control (bottom). D, Western blotting analysis of ANPEP control cells and CFL1 knockdown mutants. Total protein samples were serially diluted. Blots were probed with CFL1 antibody (top) or α-Tubulin antibody as a loading control (bottom).

Article Snippet: Human Jurkat T cells (ATCC TIB-152, clone E6-1) were cultured as described ( , ) at 37°C and 5% CO in RPMI 1640 medium with L-glutamine, without phenol red (Gibco), supplemented with 10 mM HEPES (Sigma-Aldrich), 100 U/mL penicillin and 100 μg/mL streptomycin (Gibco), and 10% heat-inactivated fetal bovine serum (Gibco).

Techniques: Western Blot, Expressing, Stable Transfection, Transduction, Construct, Control, Quantitative RT-PCR, Knockdown, Two Tailed Test

Journal: Frontiers in Oncology

Article Title: Valproate reactivates HTLV-1 tax and reduces ABCB1/MDR1 expression in PBMCs derived from ATLL patients

doi: 10.3389/fonc.2026.1721313

Figure Lengend Snippet: ATLL cells from untreated patients are intrinsically resistant to topoisomerase II inhibitors. (A, B) Jurkat and ATL-2 cell lines were treated for 48 hours with increasing concentrations of DMSO, etoposide (Eto), doxorubicin (Doxo), vinblastine (VinB), oxaliplatin (OxIP), and camptothecin (CPT). (C, D) CD8 + cell–depleted PBMC were incubated for 48 hours with increasing concentrations of Eto or Doxo, respectively. Cell viability was assessed using PrestoBlue vital dye (Invitrogen), and survival rate was calculated relative to untreated controls.

Article Snippet: Human T-cell lines Jurkat (ATCC TIB-152, Manassas, VA, USA), HuT78 (ATCC TIB-161, Manassas, VA, USA), JPX9 (RRID: CVCL_0D86, BioVector NTCC, Beijing, China), HuT-102 (ATCC TIB-162, Manassas, VA, USA), C8166 (ECACC 88051601, Salisbury, UK), and ATL-2 (-) (CVCL_A6TF, RIKEN BioResource Center, Japan) were cultured in RPMI-1640 supplemented with 10% fetal calf serum (FCS) in low-binding T75 flasks (Sarstedt AG & Co, Nürmbrecht, Germany).

Techniques: Incubation

Journal: Frontiers in Oncology

Article Title: Valproate reactivates HTLV-1 tax and reduces ABCB1/MDR1 expression in PBMCs derived from ATLL patients

doi: 10.3389/fonc.2026.1721313

Figure Lengend Snippet: Expression analysis of efflux pumps from the ATP-ABC transporter family in HTLV-1-derived cell lines. (A–E) Expression of five ATP-ABC transporter family members associated with chemoresistance was assessed by RT-qPCR in two HTLV-1-negative cell lines (HuT78, Jurkat) and three HTLV-1-derived cell lines (HuT102, C81–66, ATL-2). (F, G) Relative expression of Tax and HBZ in HuT78, Jurkat, and HTLV-1-derived cell lines was measured by RT-PCR. Statistical significance was determined by one-way ANOVA with Dunn’s multiple comparisons post-test: ns, p ≤ 0.05, ** p ≤ 0.01; *** p ≤ 0.001, **** p ≤ 0.0001.

Article Snippet: Human T-cell lines Jurkat (ATCC TIB-152, Manassas, VA, USA), HuT78 (ATCC TIB-161, Manassas, VA, USA), JPX9 (RRID: CVCL_0D86, BioVector NTCC, Beijing, China), HuT-102 (ATCC TIB-162, Manassas, VA, USA), C8166 (ECACC 88051601, Salisbury, UK), and ATL-2 (-) (CVCL_A6TF, RIKEN BioResource Center, Japan) were cultured in RPMI-1640 supplemented with 10% fetal calf serum (FCS) in low-binding T75 flasks (Sarstedt AG & Co, Nürmbrecht, Germany).

Techniques: Expressing, Derivative Assay, Quantitative RT-PCR, Reverse Transcription Polymerase Chain Reaction

Journal: Frontiers in Oncology

Article Title: Valproate reactivates HTLV-1 tax and reduces ABCB1/MDR1 expression in PBMCs derived from ATLL patients

doi: 10.3389/fonc.2026.1721313

Figure Lengend Snippet: Verapamil does not inhibit ABCB1 in ATLL primary cells. (A) Western blot of unboiled extracts from control T-cell lines HuT78 (lane 1) and Jurkat (lane 2), and HTLV-1-infected lines HUT-102 (lane 3), C8166 (lane 4), ATL-2 (lane 5). IB, immunoblot. (B) Western blot of unboiled extracts from peripheral blood mononuclear cells of four HTLV-1 asymptomatic carriers (AC; lanes 1–4) and four untreated acute ATLL patients (ATL; lanes 5–8). IB, immunoblot. (C) Densitometric quantification of ABCB1 normalized to actin using ImageJ. Data are mean ± SD; significance by unpaired t-test (p = 0.0026). (D) Calcein-AM efflux assay schematic for measuring ABCB1 activity (D) in T-cell lines (E) and HTLV-1-infected PBMCs (F) . Inhibition is expressed relative to verapamil-treated controls. Data are mean ± SD; one-way ANOVA with Dunn’s multiple-comparisons post-test: ns, not significant; *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001; ****p ≤ 0.0001.

Article Snippet: Human T-cell lines Jurkat (ATCC TIB-152, Manassas, VA, USA), HuT78 (ATCC TIB-161, Manassas, VA, USA), JPX9 (RRID: CVCL_0D86, BioVector NTCC, Beijing, China), HuT-102 (ATCC TIB-162, Manassas, VA, USA), C8166 (ECACC 88051601, Salisbury, UK), and ATL-2 (-) (CVCL_A6TF, RIKEN BioResource Center, Japan) were cultured in RPMI-1640 supplemented with 10% fetal calf serum (FCS) in low-binding T75 flasks (Sarstedt AG & Co, Nürmbrecht, Germany).

Techniques: Western Blot, Control, Infection, Activity Assay, Inhibition

Journal: Frontiers in Oncology

Article Title: Valproate reactivates HTLV-1 tax and reduces ABCB1/MDR1 expression in PBMCs derived from ATLL patients

doi: 10.3389/fonc.2026.1721313

Figure Lengend Snippet: Tax inhibits ABCB1 expression in HuT78 and JPx9T-cell lines. (A) HuT78 cells were transfected with P65-Flag, P52, SP1, or Tax-Flag plasmids. Cells were harvested 48 hours post-transfection, and ABCB1 mRNA levels were measured by RT-qPCR. Statistical significance was determined by one-way ANOVA with Dunn’s multiple comparisons post-test (ns, p ≤ 0.05, * p ≤ 0.01, ** p ≤ 0.001, *** p ≤ 0.00001). (B) Western blot analysis of Tax-Flag and ABCB1 expression, with actin as the loading control. (C) Calcein-AM efflux kinetics measuring ABCB1 activity in Hut78 cells for 8 minutes with 100 µM verapamil (green curve) as a control. (D-F) JPX9 cells (human T-cell line with inducible HTLV-1 provirus) were treated for 24 hours with 20 µM cadmium chloride (CdCl2).Tax and ABCB1 expression was measured by RT-qPCR (D) and Western blotting (E, F) Inhibition of ABCB1 efflux activity was measured using the Calcein AM assay. The percentage of inhibition was calculated relative to control cells treated with verapamil.

Article Snippet: Human T-cell lines Jurkat (ATCC TIB-152, Manassas, VA, USA), HuT78 (ATCC TIB-161, Manassas, VA, USA), JPX9 (RRID: CVCL_0D86, BioVector NTCC, Beijing, China), HuT-102 (ATCC TIB-162, Manassas, VA, USA), C8166 (ECACC 88051601, Salisbury, UK), and ATL-2 (-) (CVCL_A6TF, RIKEN BioResource Center, Japan) were cultured in RPMI-1640 supplemented with 10% fetal calf serum (FCS) in low-binding T75 flasks (Sarstedt AG & Co, Nürmbrecht, Germany).

Techniques: Expressing, Transfection, Quantitative RT-PCR, Western Blot, Control, Activity Assay, Inhibition, Calcein AM Assay